Reverse Complement of DNA Sequences

In a DNA molecule, the two antiparallel strands (positive or 5’-> 3’ and negative or 3’-> 5’) are complementary to each other

As per standard notation, we write the DNA sequence in 5’-> 3’ orientation. Therefore, to represent a sequence of the negative strand in 5’-> 3’ orientation we need to perform a reverse complementary of positive strand (e.g. reverse or left primer)

You can perform the reverse complement of DNA sequences using Python or Bash

This work is licensed under a Creative Commons Attribution 4.0 International License